AOAC ERP Gluten Assays - Dec 2018

DATA RETURN SHEET: RUN 1 (SAMPLES 1 to 21) RIDASCREEN ® Total Gluten R7041

Run 1 (samples 1 to 21)

Lab Code Date

YYYY-MM-DD

Plate layout

PREPLATE

1

2 2 3 4 5 6 7 8 9

3 2 3 4 5 6 7 8 9

4

5

6

7

1

2 and 3

4 and 5

6

7

A B C D E F G H

STD 1 STD 2 STD 3 STD 4 STD 5 STD 6

10 11 12 13 14 15 16 17

10 11 12 13 14 15 16 17

18 18 19 19 20 20 21 21

STD 1 STD 2 STD 3 STD 4 STD 5 STD 6

A STD 1 B STD 2 C STD 3 D STD 4 E STD 5

2 3 4 5 6 7 8 9

10 11 12 13 14 15 16 17

18 18 19 19 20 20 21 21

STD 1 STD 2 STD 3 STD 4 STD 5 STD 6

F

STD 6

1 1

G H

1 1

pipetting scheme for a plate with 7 strips STD Standard (for number see label on the vial) 1 to 21 sample number

Results (OD values)

OD-Values obtained (absorbance units) (platereader data) Specify plate reader

IMPORTANT REMARK

1

2

3

4

5

6

7

A B C D E F G H

If samples show OD values higher than STD 6 dilute further at 1:5 (1+4) with the following buffer - 1% Cocktail (patented), depending on the used extraction

- 3% of an 80% ethanol solution

- 96% buffer

e.g. 50 µl Cocktail, 150 µl 80% ethanol and 4800 µl buffer.

Refer to the Instructions for collaborators

Use the Excel sheet "Samples with OD > STD6" to document the analysis of further diluted samples