AOAC ERP Gluten Assays - Dec 2018

DATA RETURN SHEET: RUN 2 (SAMPLES 22 to 42) RIDASCREEN ® Total Gluten R7041

Run 2 (samples 22 to 42)

Lab Code Date

YYYY-MM-DD

Plate layout

PREPLATE

1

2

3

4

5

6

7

1

2 and 3

4 and 5

6

7

A B C D E F G H

STD 1 STD 2 STD 3 STD 4 STD 5 STD 6

23 24 25 26 27 28 29 30

23 24 25 26 27 28 29 30

31 32 33 34 35 36 37 38

31 32 33 34 35 36 37 38

39 39 40 40 41 41 42 42

STD 1 STD 2 STD 3 STD 4 STD 5 STD 6

A STD 1 B STD 2 C STD 3 D STD 4 E STD 5

23 24 25 26 27 28 29 30

31 32 33 34 35 36 37 38

39 39 40 40 41 41 42 42

STD 1 STD 2 STD 3 STD 4 STD 5 STD 6

F

STD 6

22 22

G H

22 22

pipetting scheme for a plate with 7 strips STD Standard (for number see label on the vial) 22 to 42 sample number

Results (OD values)

OD-Values obtained (absorbance units) (platereader data) Specify plate reader

IMPORTANT REMARK

1

2

3

4

5

6

7

A B C D E F G H

If samples show OD values higher than STD 6 dilute further at 1:5 (1+4) with the following buffer - 1% Cocktail (patented), depending on the used extraction

- 3% of an 80% ethanol solution

- 96% buffer

e.g. 50 µl Cocktail, 150 µl 80% ethanol and 4800 µl buffer.

Refer to the Instructions for collaborators

Use the Excel sheet "Samples with OD > STD6" to document the analysis of further diluted samples