AOAC SPIFAN Review Team Meeting Book-December 2015
2011.06 (Fol-22) w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE
Silliker Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis
Table 26: Results of the SPIFAN samples for total folate calculated as sum of the detected folate compounds and expressed as mcg/100 g of the reconstitute beverage liquid sample or RTF liquid sample. Averages and precision (as % RSD) of replicates analyzed on the same day for separate 3 days (total n=9).
REC'D AS
Analyzed AS
Av. mcg/100g RTF*
Independent Analysis Days
Sample
Matrix
Lot #/ID
Stdev %RSD
n
Breakfast Cereal Sample
Silliker LCS
LCS**
Powder
Powder
65.2
2.9
4.5
9
3
RTF Liquid RTF Liquid
1
Powder
31.8
2.5
7.9
9
3
SRM 1849A
CLC10-b
Adult Nutritional Powder Milk Protein Based
2
Powder
35.6
4.5
12.7
9
3
11750017V3
Infant Formula Powder Partially Hydrolyzed Milk Based Infant Formula Powder Partially Hydrolyzed Soy Based Adult Nutritional Powder Low Fat
RTF Liquid
3
Powder
20.0
2.5
12.5
9
3
1172572116
RTF Liquid RTF Liquid RTF Liquid RTF Liquid RTF Liquid RTF Liquid RTF Liquid RTF Liquid RTF Liquid
4
Powder
25.3
2.2
8.9
9
3
117257651Z
5
Powder
42.2
4.1
9.7
9
3
00394RF00
6
Powder
24.6
1.8
7.5
9
3
Child Formual Powder
00412RF00
7
Powder
32.6
3.0
9.3
9
3
Infant Elemental Powder
00403RF00
Infant Formula Powder Milk Based Infant Formula Powder Soy Based
8
Powder
21.5
1.5
7.0
9
3
D04HTCVV
9
Powder
22.5
2.4
10.5
9
3
E29JVLV
SPIFAN CONTROL MILK FORM
Infant Formula RTF Milk Based
10
LIQUID
19.5
2.1
10.5
9
3
Adult Nutritional RTF High Protein Adult Nutritional RTF High Fat
11
LIQUID
56.0
5.5
9.9
9
3
00414RF00
12
LIQUID
66.2
6.5
9.8
9
3
00406RF00
* Total Folate Results are calculated based on sum of all the folate compounds in the samples after the conversion of each compound as folic acid. Results are provided as mcg/g per 100 g of RTF **Total Folate Values for Cereal sample is per 100g of the solid sample The results of total folate as folic acid (sum of the detected folate compounds after calculation as folic acid) in the SPIFAN samples are presented in the above Table. The samples were analyzed in triplicates for three separate days. Averages and repeatability value/precision (%RSDr) for within day analysis are presented. The repeatability/precision (% RSDr) with one exception were less than 7% provided in the SMPR for all the SPIFAN samples for all three days of the analysis.
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