RI-ERP-FINALACTION-Recommendations

OMA 2015.13: Collaborative Study Protocol Expert Review Panel Use Only September, 2017

Food and Environmental Surfaces (2012). Table 1. Overview of Study Design

# Plates per dilution

Target * Levels

SAMPLE CODES

MATRIX

3M RAC

SMEDP/ BAM

~50 cfu/g

4** 4** 4**

2 2 2 2 2 2 2 2

Raw easy peel shrimp (50g)

Random number between 100-199

~500 cfu/g ~5000 cfu/g ~50 cfu/mL ~500 cfu/mL ~5000 cfu/mL

2 2 2 2 2 2

Pasteurized skim milk (11mL) Instant non-fat dried milk (11g)

Random number between 200-299

~50 cfu/g

Random number between 300-399

~500 cfu/g ~5000 cfu/g

2 *Actual inoculation levels may vary with a target of 0.5 to 1.0 Log 10 between levels **Two plates incubated at 32°C and two plates incubated at 35°C

difference

1.2. Study Schedule 1.2.1. One matrix will be tested each week of the study. All data should be sent to the co-Study Director by the 4 th week of study. 1.2.2. One co-Study Director will prepare the test portions and ship the test portions in leak-proof, insulated containers with ice packs via overnight courier to the collaborators on Monday. Test portion processing and analysis will begin on Wednesday for easy peel shrimp and pasteurized skim milk. Instant non-fat dried milk will be shipped on a Thursday with processing and analysis beginning on Monday. Data forms will be completed and faxed or emailed back to the co-Study Director by the following Friday.

AOAC Research Institute Expert Review Panel Use Only

Test Portion Preparation Matrices