RI-ERP-FINALACTION-Recommendations

OMA 2015.13: Collaborative Study Protocol Expert Review Panel Use Only September, 2017

To reduce the risks associated with bacterial infection and workplace contamination: • Perform 3M Petrifilm RAC Plate testing in a properly equipped laboratory under the control of a skilled microbiologist. • The user must train personnel in current proper testing techniques: for example, Good Laboratory Practices 1 , ISO 17025 2 or ISO 7218 3 . To reduce the risks associated with misinterpretation of results: • 3M has not documented 3M Petrifilm RAC Plates for use in industries other than food and beverage. For example, 3M has not documented 3M Petrifilm RAC Plates for testing water, pharmaceuticals, or cosmetics. • Do not use 3M Petrifilm RAC Plates in the diagnosis of conditions in humans or animals. • Do not use 3M Petrifilm RAC Plates for U.S.-recognized laboratory pasteurized counts. • Acceptance of the 3M Petrifilm RAC Plate method for the testing of water per an accepted local government regulation is at the sole discretion and responsibility of the end-user. • 3M Petrifilm RAC Plates do not differentiate any one microorganism strain from another. Test Kit - 3M™Petrifilm™Rapid Aerobic Count Plate Cat. #6478 3M Petrifilm Rapid Aerobic Count Plates 25/pouch, 2 pouches/box Cat. # 6479 3M Petrifilm Rapid Aerobic Count Plates 25/pouch, 20 pouches /case Additional Reagents Sterile diluent –Butterfields Phosphate Diluent (BPD) Additional Media Standard Methods Agar (SMA) Plate Count Agar (PCA) Apparatus Pipettes . –capable of pipetting 1,000µL or sterile serological pipette Sterile pipette tips. –capable of 1,000µL Stomacher® . –Seward or equivalent Filter Stomacher®bags . - Seward or equivalent 3M™Petrifilm™Flat Spreader (6425) Incubators . –Incubator capable of maintaining 32±1°C and 35±1°C, and having a solid front so as to maintain a dark interior. Refrigerator . –capable of maintaining 2-8°C, for storing the 3M Petrifilm RAC Plates Freezer . - capable of maintaining -10° to -20°C, for storing opened 3M Petrifilm RAC Plate pouches, 3M Petrifilm RAC Plates after AOAC Research Institute Expert Review Panel Use O ly