AOAC SPIFAN Stakeholder Panel Meeting Book (September 7, 2019)
Analytical Techniques: LC-Mass Spectrometry (LCMS)
LCMS: Intact protein quant
X Need HRMS due to large size of the molecule (>78 kDa)
X Most triple quadrupole instruments <1400-2000 Da
TOF, Orbitrap, etc
X
Multiple charge states
X
X Lactoferrin intact will exist in multiple charge states (mass/charge = m/z)
Requires deconvolution
X
Post Translational Modifications (PTMs)
X
Glycosylation state well documented
X
Other modifications possible
X
X Instrumentation cost high for all LCMS analysis, especially HRMS
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