AOAC SPADA VNGS - Final

Comments Received and Reconciliation

Type of comment Line #

Comment

SPADA WG Response

Technical

41 Per London Calling 2022 update from Nanopore Technologies, they will be getting rid of the FAST5 format and moving to the "pod5" format if the move has not been made already. Maybe relevant to include separately or along with FAST5 definition. 176 ‐ 178 This section is not a complete sentence. Suggest: "At a minimum, annotation information must include sample name...clinical or environmental sample." 181 Remove "human". Not sure why human needs to be specified. The requirement of English should be specific enough. 192 ‐ 193 Could add the new "pod5" Nanopore Technologies format here as well. 108 Assigning the responsibility to individual parties to avoid assignment of equivalent VNGS URIs to multiple biothreat agent VNGS is good; however, responsible parties must have the ability to verify assigned URIs do not conflict. For instance, are canonical URLs used? 88 ‐ 89 This may have been in the document and I over looked it but what standards cover Verified Next Generation Sequence (VNGS). Or does that line mean the written standards of the current document.

accepted

Editorial

accepted

Editorial

accepted

accepted

Technical

Technical

not accepted, canonical URLs do not have the same URIs.

General

We mean the current standard.

General

47, 71, 95, 142, 166, 214, 328, 352, 376

I wasn't able to read the comments due to the overlay

Our apologies.

Technical

27 Biothreat agent ‐ Add a DOD context to the definition separate from the public health concerns. 37 Coverage ‐ Add definitions for depth and breadth of coverage 231 Insert breadth of coverage with definition between (17) and (18). Make (17) depth of coverage.

accepted with modification. The literary path used in OMA Appendix O was applied. DOD does not directly specifiy a list of BSAT.

accepted accepted

Technical Technical