AOAC SPADA VNGS - Final

Ontology .—Logical structure of the terms used to describe a domain of knowledge, including both the 71

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definitions of the applicable terms and their relationships

OWL .—Web ontology language is a family of knowledge representation or ontology languages for 73

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authoring ontologies or knowledge bases

pod5. —High performance sequencing file format for nanopore reads 75

Quantification. —Determination of the amount of a biothreat agent in a sample 76

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Raw read .—Raw output of an NGS run

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Reifiable .—Capable of being made more concrete or real

Resource Description Framework (RDF) .—XML syntax for describing metadata 79

Responsible party .—Person or persons responsible for the provision of the standard requirements 80

Semantic interoperability .—Ability of data shared by systems to be understood at the level of fully 81

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defined domain concepts

Sequence length distribution .—Spread of sequence fragment read sizes in an NGS run (16). 83

Surveillance .—Close observation through microbiological sampling and analysis 84

UCS character set .—Character set encoding standard for international electronic communication 85

Universal resource identifier (URI) .—Sequence of characters, capable of uniquely identifying the thing 86

with which it is associated, within a specified context. URI is an internet protocol standard that builds on 87

the uniform resource indicator protocol by greatly expanding the set of permitted characters 88

Variant .—Single nucleotide polymorphism, insertion, or deletion occurring in one sequence but not in 89

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another

Verified .—Provision of objective evidence that a given item fulfils specified requirements 91

Verified next generation sequence (VNGS) .—Next generation nucleotide sequence that conforms with 92

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this standard

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AOAC Draft Standard – Version 09282022; Public Comment Revisions

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