SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 2500 mg / 100 ml : Trl 1

Vail #

: 38

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP009.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 7:39:21 AM : 1/23/2007 3:17:50 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP009.lcd

mV

1Det.A Ch1

3

500

1

4

5

250

2

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 1707820 1430482 3896838 1970705 1397803 439557 10843205

Height

Area %

Name

1 2 3 4 5 6 Total

15.554 19.735 20.402 21.401 22.258 25.534

301723 205609 550971 324628 249659 78451 1711041

15.750 13.192 35.938 18.175 12.891 4.054 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP009.lcd