SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 2500 mg / 100 ml : Trl 2

Vail #

: 39

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP010.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 8:25:06 AM : 1/23/2007 3:18:58 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP010.lcd

mV

1Det.A Ch1

3

500

1

4

250

5

2

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 1546771 1271665 3508268 1767374 1279368 393606 9767052

Height

Area %

Name

1 2 3 4 5 6 Total

15.463 19.621 20.302 21.325 22.189 25.490

272883 183718 491917 288311 225079 70117 1532025

15.837 13.020 35.919 18.095 13.099 4.030 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP010.lcd