SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 750 mg / 100 ml :Trl 1

Vail #

: 43

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP-II001.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 1:45:20 PM : 1/23/2007 2:55:48 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II001.lcd

mV

1Det.A Ch1

200

3

150

1

100

4

5

2

50

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.376 19.537 20.226 21.263 22.128 25.447

478462 398109 1099263 548663 394764 122771 3042031

84557 57593 153652 88994 69732 21895 476423

15.728 13.087 36.136 18.036 12.977 4.036 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II001.lcd