SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 750 mg / 100 ml :Trl 2

Vail #

: 44

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP-II002.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 2:31:04 PM : 1/23/2007 2:57:48 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II002.lcd

mV

1Det.A Ch1

200

3

150

1

100

4

5

2

50

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.372 19.532 20.223 21.260 22.126 25.448

479376 394834 1091327 541636 393611 120722 3021506

84003 57054 153105 87896 68735 21488 472281

15.865 13.067 36.119 17.926 13.027 3.995 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II002.lcd