SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 1000 mg / 100 ml : Trl 2

Vail #

: 46

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP-II004.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 4:04:51 PM : 1/23/2007 3:01:02 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II004.lcd

mV

1Det.A Ch1

3

200

1

4

5

100

2

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.382 19.532 20.221 21.257 22.124 25.447

645389 530604 1468232 731496 532618 162658 4070996

113325 76793 206318 118467 92849 29130 636882

15.853 13.034 36.066 17.968 13.083 3.996 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II004.lcd