SPDS SET 2: ASH-01

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 750 mg / 100 ml :Trl 1

Vail #

: 43

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP-II001.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 1:45:20 PM : 3/29/2007 5:01:03 PM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II001.lcd

mV

200

1Det.A Ch1

100

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Name

Area

Area %

Resolution

1 2 3 4 5 6 7 8 9

15.187 15.376 18.909 19.537 20.226 21.263 22.128 22.311 22.804 23.524 23.891 24.112 24.625 25.447

RT15.187 Withanolside IV RT18.909 Withanoside V Withaferin A Withastramonolide Withanolide A

225004 478462 3921 400277 1097206 548663 398098 43911 36992 100800 15874 17872 6589 122702 3496369

6.435 13.685 0.112 11.448 31.381 15.692 11.386 1.256 1.058 2.883 0.454 0.511 0.188 3.509 100.000

0.000 1.135 23.432

3.776 3.516 5.550 5.034 1.219 3.406 3.854 1.951 1.423 3.252 5.045

RT22.311 RT22.804 RT23.524 RT23.891 RT24.112 RT24.625 Withanolide B

10 11 12 13 14 Total

Chromatogram D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II001.lcd

mV

200

100

0

1Det.A Ch1

15.0

17.5

20.0

22.5

25.0

min

Det.A Ch1 / 227nm 1