SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 2500 mg / 100 ml : Trl 1

Vail #

: 51

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP-II009.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 7:53:34 PM : 1/23/2007 3:06:56 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II009.lcd

mV

1Det.A Ch1

3

500

1

4

5

250

2

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 1587176 1303746 3606086 1816122 1330784 404644 10048558

Height

Area %

Name

1 2 3 4 5 6 Total

15.468 19.627 20.306 21.329 22.193 25.498

278430 189091 505110 297967 232773 72505 1575875

15.795 12.974 35.887 18.073 13.244 4.027 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II009.lcd