SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera ext

Sample ID

: WS - 06 LOT 10

Vail #

: 19

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Quan006.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 11:49:50 AM : 1/23/2007 2:52:16 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan006.lcd

mV

1Det.A Ch1

400

3

300

1

200

4

5

2

100

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.540 19.720 20.389 21.393 22.250 25.528

944769 795041 2165191 1091421 755856 242624 5994902

166740 114533 306133 180160 136295 43606 947467

15.760 13.262 36.117 18.206 12.608 4.047 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan006.lcd