SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera ext

Sample ID

: WS - 05 LOT 21

Vail #

: 15

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Quan002.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 8:46:51 AM : 1/23/2007 2:47:29 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan002.lcd

mV

1Det.A Ch1

3

300

200

1

4

5

100

2

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.537 19.725 20.395 21.401 22.261 25.537

806225 637065 2532764 865621 670597 193202 5705473

142188 90668 356934 148099 120136 35413 893439

14.131 11.166 44.392 15.172 11.754 3.386 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan002.lcd