SPDS SET 2: ASH-01

Acquired by Sample Name

: Admin

: Withania somnifera ext

Sample ID

: RM - ERH - 46

Vail #

: 21

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Quan008.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 1:21:18 PM : 3/29/2007 4:45:13 PM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan008.lcd

mV

200

1Det.A Ch1

100

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Name

Area

Area %

Resolution

1 2 3 4 5 6 7 8 9

15.352 15.547 18.980 19.698 20.372 21.377 22.239 22.491 22.903 23.619 23.987 24.208 24.567 24.709 25.523

RT15.352 Withanoside-IV RT18.980 Withanoside V Withaferin A Withastramonolide Withanolide A

265649 491157 8341 473414 1250895 410796 384409 1440

7.501 13.869 0.236 13.368 35.323 11.600 10.855 0.041 1.535 1.036 1.082 0.417 0.139 0.115 2.883 100.000

0.000 1.158 21.729

4.128 3.455 5.561 5.143 1.589 2.662 4.654 2.373 1.282 2.088 0.994 5.790

RT22.491 RT22.903 RT23.619 RT23.987 RT24.208 RT24.567 RT24.709 Withanolide B

54369 36688 38306 14767

10 11 12 13 14 15 Total

4931 4074 102080 3541316

Chromatogram D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan008.lcd

mV

200

100

0

1Det.A Ch1

15.0

17.5

20.0

22.5

25.0

min

Det.A Ch1 / 227nm 1