SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera ext

Sample ID

: WS - 06 LOT 08

Vail #

: 27

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: SSR002.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 5:55:43 PM : 1/23/2007 3:28:18 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\SSR002.lcd

1Det.A Ch1

3

4

500

5

6

250

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm

Detector A Ch1 227nm Peak # Ret. Time

Area 1105227 650581 3965398 3110148 2296516 1547889 12675760

Height

Area %

Name

1 2 3 4 5 6 Total

15.435 19.598 20.278 21.305 22.169 25.476

189293 93589 571459 521874 392754 269117 2038086

8.719 5.132

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

31.283 24.536 18.117 12.211 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\SSR002.lcd