SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera ext

Sample ID

: WS - 05 LOT 20

Vail #

: 26

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: SSR001.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 5:10:00 PM : 1/23/2007 3:26:52 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\SSR001.lcd

1Det.A Ch1

3

4

400

5

300

6

200

1

100

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.520 19.675 20.352 21.366 22.231 25.528

683387 277532

114330 39535 447747 438803 333647 250635 1624696

6.802 2.762

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

3068219 2594887 1985457 1438005 10047486

30.537 25.826 19.761 14.312 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\SSR001.lcd