SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera ext

Sample ID

: RM - RD / 1162

Vail #

: 25

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: SR004.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 4:24:14 PM : 1/23/2007 3:25:44 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\SR004.lcd

1Det.A Ch1

3

250

4

5

200

6

150

1

100

2

50

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.540 19.691 20.366 21.374 22.236 25.525

544320 462387 1782419 1276721 1277510 1071449 6414805

93415 66000

8.485 7.208

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

259284 218915 215493 186725 1039832

27.786 19.903 19.915 16.703 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\SR004.lcd