SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withanolides : std mix

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\SM-01-Rep1

Vial No

: 10001

Injection Volume

: 20

Date & Time

: 4/3/07 7:44:32 PM

0.8

0.6

0.4

Volts

23.892

23.033

22.058

27.008

0.2

17.233

21.400

0.0

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.233 21.400 22.058 23.033 23.892 27.008

Withanoside IV Withanoside V Withaferin A

575205 274773 1813207 1788787 1849418 1522047

7.35 3.51

23.18 22.86 23.64 19.45

Withastramonolide

Withanolide A Withanolide B

Totals

7823437

100.00