SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withanolides : std mix

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\SM-01-Rep3

Vial No

: 10001

Injection Volume

: 20

Date & Time

: 4/3/07 9:16:17 PM

0.8

0.6

0.4

Volts

23.892

23.008

22.017

26.967

0.2

17.217

21.483

0.0

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.217 21.483 22.017 23.008 23.892 26.967

Withanoside IV Withanoside V Withaferin A

559628 265630 1788944 1756420 1809961 1485590

7.30 3.46

23.34 22.91 23.61 19.38

Withastramonolide

Withanolide A Withanolide B

Totals

7666173

100.00