SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : WS - 06 lot 08

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S3-Rep2

Vial No

: 10004

Injection Volume

: 20

Date & Time

: 4/4/07 5:40:51 AM

0.5

0.4

22.025

0.3

17.217

23.017

Volts

0.2

23.908

21.500

0.1

26.975

0.0

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.217 21.500 22.025 23.017 23.908 26.975

Withanoside IV Withanoside V Withaferin A

1209175 811366 2109930 1191675 1088863 267000

18.11 12.15 31.60 17.84 16.31

Withastramonolide

Withanolide A Withanolide B

4.00

Totals

6678009

100.00