SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : WS - 06 lot 10

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S4-Rep3

Vial No

: 10005

Injection Volume

: 20

Date & Time

: 4/4/07 8:44:13 AM

0.4

22.050

0.3

17.233

23.033

0.2

Volts

23.917

21.475

0.1

27.017

0.0

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.233 21.475 22.050 23.033 23.917 27.017

Withanoside IV Withanoside V Withaferin A

1165114 763045 1917315 1075780 968374 245187

18.99 12.44 31.25 17.54 15.78

Withastramonolide

Withanolide A Withanolide B

4.00

Totals

6134815

100.00