SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : WS - 05 lot 20

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S1-Rep2

Vial No

: 10002

Injection Volume

: 20

Date & Time

: 4/4/07 10:15:57 AM

0.15

22.083

0.10

Volts

17.233

23.917

23.050

0.05

21.408

27.033

0.00

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.233 21.408 22.083 23.050 23.917 27.033

Withanoside IV Withanoside V Withaferin A

286199 180229 927184 300018 321506

13.79

8.69

44.69 14.46 15.49

Withastramonolide

Withanolide A Withanolide B

59796

2.88

Totals

2074932

100.00