SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : WS - 05 lot 21

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S2-Rep1

Vial No

: 10003

Injection Volume

: 20

Date & Time

: 4/4/07 11:47:39 AM

0.20

0.15

17.225

0.10

Volts

23.033

23.908

21.408

0.05

27.025

0.00

22.058

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.225 21.408 22.058 23.033 23.908 27.025

Withanoside IV Withanoside V Withaferin A

588934 373582 1403133 496352 465701

17.19 10.90 40.95 14.49 13.59

Withastramonolide

Withanolide A Withanolide B

98415

2.87

Totals

3426117

100.00