SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : RD - 1162 : RM

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S7-Rep3

Vial No

: 10008

Injection Volume

: 20

Date & Time

: 4/4/07 1:51:37 AM

0.150

0.125

17.217

0.100

0.075

Volts

21.500

0.050

23.900

23.008

0.025

26.967

0.000

22.017

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.217 21.500 22.017 23.008 23.900 26.967

Withanoside IV Withanoside V Withaferin A

503502 296939 863184 241291 255090

22.88 13.49 39.23 10.97 11.59

Withastramonolide

Withanolide A Withanolide B

40438

1.84

Totals

2200444

100.00