SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : ERH - 046 : RM

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S8-Rep1

Vial No

: 10009

Injection Volume

: 20

Date & Time

: 4/4/07 2:37:28 AM

0.25

0.20

0.15

17.225

Volts

0.10

21.508

23.017

23.908

0.05

26.967

0.00

22.017

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.225 21.508 22.017 23.017 23.908 26.967

Withanoside IV Withanoside V Withaferin A

657778 517598 1338712 492426 444196 113041

18.46 14.52 37.56 13.82 12.46

Withastramonolide

Withanolide A Withanolide B

3.17

Totals

3563751

100.00