SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : ERH - 046 : RM

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S8-Rep2

Vial No

: 10009

Injection Volume

: 20

Date & Time

: 4/4/07 3:23:20 AM

0.25

0.20

0.15

17.225

Volts

0.10

21.500

23.017

23.900

0.05

26.975

0.00

22.025

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.225 21.500 22.025 23.017 23.900 26.975

Withanoside IV Withanoside V Withaferin A

646445 509223 1318826 485660 438254 111940

18.42 14.51 37.57 13.84 12.48

Withastramonolide

Withanolide A Withanolide B

3.19

Totals

3510348

100.00