SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : ERH - 046 : RM

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S8-Rep3

Vial No

: 10009

Injection Volume

: 20

Date & Time

: 4/4/07 4:09:12 AM

0.25

0.20

0.15

17.217

Volts

0.10

21.492

23.008

23.900

0.05

26.967

0.00

22.017

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.217 21.492 22.017 23.008 23.900 26.967

Withanoside IV Withanoside V Withaferin A

654341 513695 1323105 490071 442221 112454

18.51 14.53 37.42 13.86 12.51

Withastramonolide

Withanolide A Withanolide B

3.18

Totals

3535887

100.00