SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : VI : Soln stab:24hrs

Vail #

: 7

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Std-ss01.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 3:26:39 AM : 1/20/2007 5:04:14 PM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Std-ss01.lcd

1Det.A Ch1

4

100

6

5

3

75

50

1

25

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.506 19.685 20.357 21.366 22.228 25.515

167920 152720 576614 642763 555790 557192 2653000

26838 21510 84572 107132 91881 96219 428151

6.329 5.756

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.734 24.228 20.949 21.002 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Std-ss01.lcd