SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera ext : WS - 05 LOT 21 :Soln stab

Sample ID

Vail #

: 15

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: spl-SS01.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 9:10:52 AM : 1/23/2007 3:20:28 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\spl-SS01.lcd

mV

1Det.A Ch1

3

300

200

1

4

5

100

2

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.440 19.605 20.287 21.310 22.174 25.479

824770 629870 2544347 863998 687063 192537 5742585

144847 90882 356312 147275 120359 35181 894855

14.362 10.968 44.307 15.045 11.964 3.353 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\spl-SS01.lcd