SPDS SET 2: ASH-01

Possible matrix: Other secondary metabolites like alkaloids, phytosterols, proteins, carbohydrate, and excipients like Colloidal silicon dioxide, Light magnesium carbonate or any other inorganic salts .

3.3) Equipments:

3.3.1) Analytical Weighing Balance: Balance Name

Instrument Code

Description

Sartorius-BP-211D

QCD/CIF/EB/01

Weighing capacity 1mg to 210gm (max) with 0.01mg accuracy

Sartorius-CP-245D

QCD/AHP/EB/01 Weighing capacity 1mg to 220gm (max) with 0.1mg accuracy

3.3.2) HPLC: System

Instrumental code

Description

HPLC

QCD/CIF/HPLC/03

SHIMADZU LC 2010 A with system controller unit, degasser unit, low pressure gradient unit up to 4 solvents, pump unit with seal unit mechanism, mixer, auto sampler, column oven, UV-VIS detector, LC solution Shimadzu LC10A system equipped with binary LC10ADVP pump, with SIL10AD vp auto sampler, SPD-M 10A vp Photo Diode Array Detector, SCL 10AVP system controller, CTO 10AVP column oven in combination with Class VP software version 6.03

HPLC

QCD/CIF/HPLC/02

3.3.3) Column: Column

Column code

Column No. 625381

Description

ODS C 18 Silica

QC-18-59

Merck kGaA, Hibar ® RT250-4.9; Prepacked Column; Cat1.01886; Lichrospher® 100; RP-18(5µm); Lot L56139833; No. 625381

C 18

QC-18-57

359208-12

Phenomenex, Luna 5 µ C 18 (2)100 A, 250 X 4.60 mm, 5 microns Part No. :00G-4252- E0 No. 359208-12

C 18

QC-18-61

S.N.0170100159

Grace Smart RP 18 5µ Lot No.39/085 Col. No. S.N.0170100159 PN 5138810

3.3.4) Sonicator:

System

Instrument Code QCD/AHP/SON/01

Description

SIDILU UTRASONICS SONICATOR

50 frequency/sec, Ultrasonic frequency 37±3,230 Volt, adjust for 1-9 min +] HF-frequency 35KHz,adjust for 1-15 min 230Volt §

BANDELIN SONOREX

QCD/CIF/SON/01

1$785$/ 5(0(',(6

3DJH RI