SPDS SET 2: ASH-01

Chromatogram – 3: Raw material chromatogram

When analysed using PDA detector the retention times of Withanoside IV, Withanoside V, Withaferin A, 12-Deoxy withastramonolide, Withanolide A and Withanolide B were about 16.373, 20.565, 21.376, 22.368, 23.211 and 26.475 respectively (Refer chromatogram- 4,5,6,7,8,9,10 & 11). The PDA spectra of all the withanolides peaks in the sample are

identical to that of the respective standards. The purity of peaks due to all the withanolides

in the sample as well as standard are >0.98. The sample chromatogram has many other peaks than six withanolides.

Chromatogram – 4: Standard Withanoside IV

Chromatogram – 5: Standard Withanoside V

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3DJH RI