SPDS SET 2: ASH-01

Table-3: Data from sample chromatogram

Substance

Compound

UV detector

PDA detector

Retention time (minutes)

Relative retention time

Retention time (minutes)

Relative retention time

Standard mix

Withanoside IV

15.516

0.698

16.373

0.705

Withanoside V

19.692

0.885

20.565

0.886

Withaferin A

20.366

0.916

21.376

0.921

12-Deoxy withastramonolide

21.379

0.961

22.368

0.964

Withanolide A

22.242

1.0

23.211

1.0

Withanolide B

25.539

1.148

26.475

1.141

Extract

Withanoside IV

15.376

0.695

16.576

0.713

Withanoside V

19.537

0.883

20.672

0.889

Withaferin A

20.226

0.914

21.440

0.922

12-Deoxy withastramonolide

21.263

0.961

22.400

0.964

Withanolide A

22.128

1.0

23.243

1.0

Withanolide B

25.447

1.149

26.496

1.140

Raw material

Withanoside IV

15.547

0.699

16.576

0.713

Withanoside V

19.698

0.886

20.661

0.889

Withaferin A

20.372

0.916

21.440

0.922

12-Deoxy withastramonolide

21.377

0.961

22.389

0.963

Withanolide A

22.239

1.0

23.243

1.0

Withanolide B

25.523

1.148

26.496

1.140

1$785$/ 5(0(',(6

3DJH RI