SPDS SET 2: ASH-01

4.1.6) Conclusion: The difference in the retention time of withanolides indicates the method is capable of separating the individual withanolides from the other.

The similarity in the PDA spectra of the peaks of withanolides obtained with the sample and

the standard indicate the peak purity.

From the above data it can be concluded that the method is very specific for the estimation

of withanolides and there is no interference from any other compounds present in the extract.

Individual chromatogram of standards, standard mix chromatogram and

Enclosure-1:

chromatogram of methanol ( 8 pages) Enclosure-2: Sample chromatograms (2 pages)

Enclosure-3: Chromatogram of individual standard in a PDA detector (6 pages) and Sample chromatograms for extract and raw material (2 pages)

Enclosure-4: PDA spectrum and Peak purity spectrum (6 pages)

1$785$/ 5(0(',(6

3DJH RI