SPDS SET 2: ASH-01

PEAK AREA OF WITHANOSIDE IV

Table-11

Withanoside IV (mcg/ml)

Different Concentrations

Dilution -6 (20.64)

Dilution -5 (41.29)

Dilution -4 (82.58)

Dilution -3 (165.15)

Dilution -2 (330.30)

Dilution -1 (660.60)

S. solution (1321.20)

Replicate – 1

166047

329878

651382

1275513

2493967

4815460

9597190

Replicate –2

166038

330019

651009

1278440

2493747

4811411

9587035

Replicate –3

166015

329943

651630

1269142

2491031

4819826

9584608

Replicate –4

166025

329827

649769

1278566

2492809

4815725

9588582

Replicate – 5

165993

329140

651869

1279224

2497552

4818317

9579369

Mean

166024

329761

651132

1276177

2493821

4816148

9587357

7290.57

7256.55

Response factor Area / concentration Mean (Dilution 2 to 6) RSD (Dilution 2 to 6)

8042.32

7986.95

7885.34

7727.38

7550.17

7838.43

2.29

LINEARITY GRAPH PEAK AREA Vs CONCENTRATION

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3DJH RI