SPDS SET 2: ASH-01

PEAK AREA OF WITHAFERIN A

Table-13

Withaferin A (mcg/ml)

Different Concentrations

Dilution -6 (23.05)

Dilution -5 (46.09)

Dilution -4 (92.19)

Dilution -3 (184.39)

Dilution -2 (368.78)

Dilution -1 (737.55)

S. solution (1475.10)

Replicate – 1

573810

1134850

2231492

4332623

8325259

14666731

21469032

Replicate –2

573224

1134742

2227105

4337796

8322031

14756049

21438480

Replicate –3

573669

1134589

2228288

4310236

8317825

14770374

21482780

Replicate –4

573097

1134172

2211324

4335168

8328440

14788044

21438918

Replicate – 5

572942

1131663

2224755

4340882

8338317

14782253

21442185

Mean

573348

1134003

2224593

4331341

8326374

14752690

21454279

22578.47

20002.29

14544.29

Response factor Area / concentration Mean (Dilution 3 to 6) RSD (Dilution 3 to 6)

24875.80

24600.44

24129.54

23490.43

24274.053

2.16

LINEARITY GRAPH PEAK AREA Vs CONCENTRATION

17500000

15000000

12500000

10000000

7500000

Area

5000000

2500000

0 100 200 300 400 500 600 700 800 0 Concentrationof Withaferin-A (mcg/ml)

Best-fit values Slope

19900 ± 246.6 408700 ± 85730 -20.54 0.00005026 19390 to 20400 233200 to 584300 -36.38 to -5.052

Y-intercept when X=0.0 X-intercept when Y=0.0 1/slope 95% Confidence Intervals Slope Y-intercept when X=0.0 X-intercept when Y=0.0

Goodness of Fit r² Sy.x Is slope significantly non-zero? F DFn, DFd P value Deviation from zero? Data Number of X values Maximum number of Y replicates Total number of values Number of missing values

0.9957 337100

6509 1.000, 28.00 < 0.0001 Significant

6 5 30 0

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