SPDS SET 2: ASH-01

PEAK AREA OF 12-DEOXY WITHASTRAMONOLIDE

Table-14

12-Deoxy withastramonolide (mcg/ml)

Different Concentrations

Dilution -6 (22.21)

Dilution -5 (44.41)

Dilution -4 (88.83)

Dilution -3 (177.65)

Dilution -2 (355.30)

Dilution -1 (710.60)

S. solution (1421.20)

Replicate – 1

635487

1256078

2472835

4800626

9145644

14809446

18080882

Replicate –2

634565

1254992

2469751

4809678

9138372

14845283

18085009

Replicate –3

634904

1256714

2471386

4778467

9140140

14833377

18068450

Replicate –4

634821

1256803

2448908

4808531

9151223

14874117

18045786

Replicate – 5

634615

1254538

2471248

4813165

9156566

14859008

17998894

Mean

634878

1255825

2466826

4802093

9146389

14844246

18055804

25742.72

20889.74

12704.62

Response factor Area / concentration Mean (Dilution 3 to 6) RSD (Dilution 3 to 6)

28590.08

28276.39

27771.75

27031.20

27917.36

2.11

LINEARITY GRAPH PEAK AREA Vs CONCENTRATION

17500000

15000000

12500000

10000000

7500000

Area

5000000

2500000

0 100 200 300 400 500 600 700 800 0 Concentration of 12-Deoxy withastramonolide

Best-fit values Slope

20790 ± 465.2 678600 ± 155800 -32.65 0.00004811 19830 to 21740 359500 to 997800 -60.74 to -5.678

Y-intercept when X=0.0 X-intercept when Y=0.0 1/slope 95% Confidence Intervals Slope Y-intercept when X=0.0 X-intercept when Y=0.0

Goodness of Fit r² Sy.x Is slope significantly non-zero? F DFn, DFd P value Deviation from zero? Data Number of X values Maximum number of Y replicates Total number of values Number of missing values

0.9862 612700

1996 1.000, 28.00 < 0.0001 Significant

6 5 30 0

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