SPDS SET 2: ASH-01

PEAK AREA OF WITHANOLIDE B

Table-16

Withanolide B (mcg/ml)

Different Concentrations

Dilution -6 (21.09)

Dilution -5 (42.19)

Dilution -4 (84.39)

Dilution -3 (168.79)

Dilution -2 (337.59)

Dilution -1 (675.18)

S. solution (1350.36)

Replicate – 1

553596

1095189

2157826

4196971

7938851

12728719

12264511

Replicate –2

552800

1094573

2156794

4205282

7947620

12721830

12206630

Replicate –3

552601

1096209

2156648

4174239

7944196

12736068

12166317

Replicate –4

552865

1094840

2139922

4205370

7953428

12723957

12126384

Replicate – 5

552430

1093649

2158084

4205743

7963457

12720153

12051739

Mean

552858

1094892

2153855

4197521

7949510

12726145

12163116

23547.83

18848.52

9007.31

Response factor Area / concentration Mean (Dilution 3 to 6) RSD (Dilution 3 to 6)

26202.60

25946.08

25520.36

24867.57

25634.15

1.97

LINEARITY GRAPH PEAK AREA Vs CONCENTRATION

1000000 2000000 3000000 4000000 5000000 6000000 7000000 8000000 9000000 10000000 11000000 12000000 13000000 14000000

Area

0 100 200 300 400 500 600 700 800 0 Concentration of Withanolide B (mcg/ml)

Best-fit values Slope

18750 ± 452.1 625700 ± 143900 -33.37 0.00005334 17820 to 19670 331000 to 920400 -62.24 to -5.755

Y-intercept when X=0.0 X-intercept when Y=0.0 1/slope 95% Confidence Intervals Slope Y-intercept when X=0.0 X-intercept when Y=0.0

Goodness of Fit r² Sy.x Is slope significantly non-zero? F DFn, DFd P value Deviation from zero? Data Number of X values Maximum number of Y replicates Total number of values Number of missing values

0.9840 565800

1719 1.000, 28.00 < 0.0001 Significant

6 5 30 0

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