SPDS SET 2: ASH-01

4.2.6) Conclusion: Correlation Coefficient (r 2 ) for Withanoside IV was found to be 0.9999 Correlation Coefficient (r 2 ) for Withanoside V was found to be 0.9999 Correlation Coefficient (r 2 ) for Withaferin A was found to be 0.9957 Correlation Coefficient (r 2 ) for 12 Deoxy Withastramonolide was found to be 0.9862

Correlation Coefficient (r 2 ) for Withanolide A was found to be 0.9917 Correlation Coefficient (r 2 ) for Withanolide B was found to be 0.9840

Correlation coefficient (r 2 ) found to be >0.99 for both the analytes indicating the linearity and the

method is linear between the below concentrations.

Withanoside IV – 20. 6 and 330.0 mcg

Withanoside V – 18.7 and 300.15 mcg Withaferin A – 23.0 and 184.0 mcg

12 Deoxy Withastramonolide – 22.0 and 177.0mcg Withanolide A – 21.0 and 172.0 mcg

Withanolide B – 21.0 and 169.0 mcg

Enclosure-5: Refer standard chromatograms (35 Pages)

1$785$/ 5(0(',(6

3DJH RI