SPDS SET 2: ASH-01

Table-19: Peak area of Withanoside IV in sample (B. No. WS/06Lot10) Replicate Conc-1 (753.6 mg/100ml) Conc-2 (1503.2 mg/100ml) Conc-3 (2096.0 mg/100ml) Replicate – 1 478028 949957 1320439

Replicate - 2

479376

949797

1333648

Mean

478702

949877

1327044

RSD

0.14

0.01

0.50

Table-20: Peak area of Withanoside V

Withanoside V (mcg/ml)

Different Concentrations

Dilution -6 (18.76)

Dilution -5 (37.52)

Dilution -4 (75.04)

Dilution -3 (150.08)

Dilution -2 (300.15)

Dilution -1 (600.30)

S. solution (1200.60)

151294

298008

586819

1146136

2238822

4334899

8731747

Replicate – 1

151121

297953

585559

1146843

2237319

4327804

8721728

Replicate – 2

151156

298036

585906

1141122

2243282

4330341

8719358

Replicate – 3

151277

297352

581818

1152990

2237337

4338646

8718285

Replicate – 4

150985

297517

584964

1151745

2248262

4345473

8714428

Replicate – 5

151167

297773

585013

1147767

2241004

4335433

8721109

Mean

0.08

0.11

0.33

0.42

0.21

0.16

0.07

RSD

Table-21: Peak area of Withanoside V in sample (B. No. WS/06Lot10) Replicate Conc-1 (753.6 mg/100ml) Conc-2 (1503.2 mg/100ml) Conc-3 (2096.0 mg/100ml) Replicate - 1 401702 793298 1090118

Replicate - 2

394834

783540

1102054

Mean

398268

788419

1096086

RSD

0.86

0.62

0.54

Table-22: Peak area of Withaferin A

Withaferin A (mcg/ml)

Different Concentrations

Dilution -6 (23.05)

Dilution -5 (46.09)

Dilution -4 (92.19)

Dilution -3 (184.39)

Dilution -2 (368.78)

Dilution -1 (737.55)

S. solution (1475.10)

573810

1134850

2231492

4332623

8325259

14666731

21469032

Replicate – 1

573224

1134742

2227105

4337796

8322031

14756049

21438480

Replicate – 2

573669

1134589

2228288

4310236

8317825

14770374

21482780

Replicate – 3

573097

1134172

2211324

4335168

8328440

14788044

21438918

Replicate – 4

572942

1131663

2224755

4340882

8338317

14782253

21442185

Replicate – 5

573348

1134003

2224593

4331341

8326374

14752690

21454279

Mean

0.07

0.05

0.03

0.06

0.06

0.34

0.10

RSD

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3DJH RI