SPDS SET 2: ASH-01

Table-37: Retention time of 12-Deoxy Withastramonolide in sample (B. No. WS/06Lot10) Replicate Conc-1 (753.6 mg/100ml) Conc-2 (1503.2 mg/100ml) Conc-3 (2096.0 mg/100ml) Replicate - 1 21.378 21.261 21.330 Replicate - 2 21.260 21.262 21.284 Mean 21.319 21.262 21.307 RSD 0.28 0.002 0.11

Table-38: Retention time of Withanolide A

Withanolide A (mcg/ml)

Different Concentrations

Dilution -6 (21.59)

Dilution -5 (43.19)

Dilution -4 (86.38)

Dilution -3 (172.76)

Dilution -2 (345.51)

Dilution -1 (691.02)

S. solution (1382.04)

Replicate – 1

22.219

22.216

22.255

22.253

22.225

22.391

22.219

Replicate – 2

22.209

22.226

22.23

22.23

22.225

22.227

22.224

Replicate – 3

22.224

22.225

22.245

22.241

22.206

22.221

22.229

Replicate – 4

22.206

22.229

22.231

22.227

22.247

22.223

22.224

Replicate – 5

22.221

22.224

22.235

22.241

22.364

22.213

22.228

Mean

22.216

22.224

22.239

22.062

22.253

22.255

22.225

RSD

0.04

0.02

0.05

0.05

0.29

0.34

0.02

Table-39: Retention time of Withanolide A in sample (B. No. WS/06Lot10) Replicate Conc-1 (753.6 mg/100ml) Conc-2 (1503.2 mg/100ml) Conc-3 (2096.0 mg/100ml) Replicate - 1 22.234 22.126 22.192

22.126

22.128

22.149

Replicate - 2

Mean

22.180

22.127

22.171

RSD

0.24

0.005

0.097

PEAK AREA OF WITHANOLIDE B

Table-40: Retention time of Withanolide B

Withanolide B (mcg/ml)

Different Concentrations

Dilution -6 (21.09)

Dilution -5 (42.19)

Dilution -4 (86.38)

Dilution -3 (172.76)

Dilution -2 (345.51)

Dilution -1 (675.18)

S. solution (1350.36)

Replicate – 1

25.517

25.509

25.544

25.53

25.514

25.639

25.521

Replicate – 2

25.511

25.526

25.52

25.521

25.514

25.525

25.527

Replicate – 3

25.515

25.516

25.538

25.536

25.512

25.516

25.522

Replicate – 4

25.51

25.526

25.529

25.514

25.551

25.521

25.516

Replicate – 5

25.509

25.52

25.53

25.529

25.625

25.513

25.52

Mean

25.512

25.519

25.532

25.526

25.543

25.543

25.521

RSD

0.01

0.03

0.04

0.03

0.19

0.21

0.02

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