SPDS SET 2: ASH-01

Table-41: Retention time of Withanolide B in sample (B. No. WS/06Lot10) Replicate Conc-1 (753.6 mg/100ml) Conc-2 (1503.2 mg/100ml) Conc-3 (2096.0 mg/100ml) Replicate - 1 25.530 25.449 25.499

25.448

25.450

25.476

Replicate - 2

Mean

25.489

25.450

25.488

RSD

0.16

0.0020

0.06

4.3.10) Conclusion:

1) Relative standard deviation for the mean values of retention times for Withanoside IV, Withanoside V, Withaferin A, 12-Deoxy withastramonolide, Withanolide A and Withanolide

B in standard mix dilution is < 0.1%.

2) Relative standard deviation for the mean values of retention times for Withanoside IV,

Withanoside V, Withaferin A, 12-Deoxy withastramonolide, Withanolide A and Withanolide B in sample is < 0.1%.

For Standard chromatograms refer Enclosure-5

Enclosure – 6: Sample chromatograms (6 Pages)

1$785$/ 5(0(',(6

3DJH RI