SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : I

Vail #

: 12

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin028.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 9:12:30 PM : 1/23/2007 2:27:45 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin028.lcd

mV

1Det.A Ch1

4

5

2000

6

3

1500

1000

1

2

500

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 4819826 4330341 14770374 14833377 13484780 12736068 64974766

Height

Area %

Name

1 2 3 4 5 6 Total

15.502 19.687 20.344 21.363 22.221 25.516

765666 614859 2008055 2176286 2067542 2030965 9663373

7.418 6.665

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

22.732 22.829 20.754 19.602 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin028.lcd