SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : II

Vail #

: 11

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin021.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 3:52:03 PM : 1/23/2007 2:16:33 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin021.lcd

mV

1Det.A Ch1

1500

4

6

5

3

1000

500

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 2493967 2238822 8325259 9145644 7955602 7938851 38098145

Height

Area %

Name

1 2 3 4 5 6 Total

15.489 19.676 20.337 21.360 22.225 25.514

397258 317354 1206681 1496756 1298608 1360472 6077129

6.546 5.876

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.852 24.005 20.882 20.838 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin021.lcd