SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : II

Vail #

: 11

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin023.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 5:23:35 PM : 1/23/2007 2:20:43 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin023.lcd

mV

1Det.A Ch1

1500

4

6

5

3

1000

500

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 2491031 2243282 8317825 9140140 7958121 7944196 38094594

Height

Area %

Name

1 2 3 4 5 6 Total

15.485 19.648 20.314 21.341 22.206 25.512

396845 318405 1206269 1496559 1300544 1358501 6077124

6.539 5.889

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.835 23.993 20.890 20.854 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin023.lcd