SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : II

Vail #

: 11

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin025.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 6:55:09 PM : 1/23/2007 2:22:35 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin025.lcd

mV

1Det.A Ch1

1500

4

6

5

3

1000

500

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 2497552 2248262 8338317 9156566 7973789 7963457 38177944

Height

Area %

Name

1 2 3 4 5 6 Total

15.680 19.877 20.525 21.510 22.364 25.625

396047 320496 1230031 1511496 1319908 1360236 6138214

6.542 5.889

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.841 23.984 20.886 20.859 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin025.lcd