SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : III

Vail #

: 10

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin020.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 3:06:16 PM : 1/23/2007 2:13:49 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin020.lcd

mV

1Det.A Ch1

4

750

6

5

3

500

250

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 1279224 1151745 4340882 4813165 4149692 4205743 19940451

Height

Area %

Name

1 2 3 4 5 6 Total

15.502 19.688 20.355 21.376 22.241 25.529

202273 163396 634261 801113 684028 728042 3213114

6.415 5.776

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.769 24.138 20.810 21.092 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin020.lcd